Topoglidis Manos

Electrochemical Biosensors based on Mesoporous Metal Oxide Films

Work has concentrated on the preparation and use of mesoporous metal oxide films such as TiO2, ZnO, SnO2 and ZnO nanorods for the immobilization of active redox enzymes at their surfaces. The adsorption, diffusion and reaction processes are investigated in detail. Emphasis is given on the design and construction of electrochemical biosensors incorporating fully functional biological molecules. Electrochemical (Cyclic voltammetry and Chronoamperometry) and spectroelectrochemical techniques are employed to study the direct electron transfer between the immobilized protein and the mesoporous electrodes. The aim is to investigate under which conditions the immobilized proteins ideally still function as in vivo.

In addition we prepare hybrid films by mixing the TiO2 paste with carbon nanotubes (CNTs) or by incorporating silver nanoparticles on the TiO2 film surface in order to improve the electrochemical properties of the films and to develop more sensitive electrochemical biosensors for sensing small molecules such as hydrogen peroxide (H2O2) and glucose.


Representative publications:

  • “Factors which affect protein adsorption on nanostructured titania films. A novel electrochemical application to sensing,” E. Topoglidis, C.J. Campbell, A.E.G. Cass, and J.R. Durrant, Langmuir 17, 7899-7906 (2001).
  • “Direct electrochemistry and nitric oxide interaction of heme proteins adsorbed on nanocrystalline tin oxide electrodes,” E. Topoglidis, Y. Astuti, F. Duriaux, M. Gratzel and J.R. Durrant, Langmuir 17, 6894-6900 (2003).
  • “Nitric oxide biosensors based on the immobilization of Hemoglobin on mesoporous titania electrodes,” E. Topoglidis, C.J. Campbell, A.E.G. Cass and J.R. Durrant, Electroanalysis 18, 882-887 (2006).
  • “Interfacial electron transfer on cytochrome-c sensitised conformally coated mesoporous TiO2 films,” E. Topoglidis, T. Lutz, J.R. Durrant and E. Palomares, Bioelectrochemistry 74, 142-148 (2008).
  • “Direct Spectroelectrochemistry of Peroxidases immobilized on nanostructured metal oxide electrodes: towards reagentless hydrogen peroxide sensing,” Y. Astuti, E. Topoglidis, A.E.G. Cass and J.R. Durrant, Analytica Chimica Acta 648, 2-6 (2009).


Contact Details

(+30) 2610 996322

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